英语翻译High performance liquid chromatographyHPLC determination was performed with a Shimadzu ModelLC-6A liquid chromatograph supplied with a Reodyne Model7125 manual injector with a 20-μl loop.The detector wasShimadzu Model SPD-6A UV spectrome

英语翻译
High performance liquid chromatography
HPLC determination was performed with a Shimadzu Model
LC-6A liquid chromatograph supplied with a Reodyne Model
7125 manual injector with a 20-μl loop.The detector was
Shimadzu Model SPD-6A UV spectrometer.The Thermo Separation
Products integrator Model SP 4400 was used for data acquisition
and recording.Prior to use,the eluents were filtrated through
0.45 μm.filters (Sartorius) and degassed under the vacuum.The
column was reversed-phase Hypersil ODS (150×4.6 mm; participle
size 5 μm.) with a Hypersil ODS guard column (Supelco Inc.).
The beer samples were analysed for biogenic amines according to
the modified Geiger method [18].The elution program consisted
of a gradient system with the following solvents:(A) 0.005 M.
ammonium acetate buffer solution adjusted to pH 4.3 with glacial
acetic acid; (B) methanol.After loading the sample,analysis started
with a methanol (B) gradient in buffer A from 65% to 80% for
20 min.and then with isocratic elution of 100% methanol for
10 min.Finally,the column was equilibrated with an isocratic elution
of 65% methanol in buffer A for 10 min.
The flow rate was 1 mL/min.The eluted dansylamines were
detected by monitoring the UV absorbency at 340 nm.
Results
Twenty-seven samples of popular Polish beers were tested
by HPLC method.Eight biogenic amines:monoamines
(TRP,PHM,HIS,TYR),diamines (PUT,CAD),
polyamines (SPD,SPM) and polyamine-derived secondary
metabolites (PRP,PIR) were investigated in the beer
samples.The gradient elution of methanol in ammonium
acetate buffer allowed elution of all the amines in less
than 30 min.In the tested range of biogenic amine concentration
from 0.2 to 10.0 mg/L the correlation coefficient
between peak area and concentration of each standard
amine was higher than 0.99 for every standard
curve.The average recoveries of the amines ranged from
81.6% to 104.1% with the lowest value for spermine and
the highest for spermidine.The quantification limits
were established asranged from 0.19 mg/L for spermidine to 0.49 mg/L for2-phenylethylamine three times the detection limit and
and后再加一句ranged from 0.19 mg/L for spermidine to 0.49 mg/L for
2-phenylethylamine.

高效液相色谱法
高效液相色谱法测定与Shimadzu模型进行了
LC-6A高效液相色谱仪配置Reodyne模型
7125手册和20-μl喷油器循环.这个探测器
紫外分光计SPD-6A Shimadzu模型.这个热分离
产品积分模型被用来四千四百藻数据采集
和记录.使用前,eluents少了
μm.0.45过滤(生产)和degassed真空.这个
ODS柱(150×反Hypersil 46毫米;分词
5μm大小.)(Supelco ODS柱Hypersil护卫股份有限公司).
分析了啤酒样品为生物胺根据
修正法[d]盖.其中的教材
一个渐变系统与下列溶剂:(一)0.005).
醋酸铵缓冲溶液调节pH 4.3和冰川
醋酸;(2)甲醇.装船后,分析了样本
用甲醇(B)梯度的缓冲液,由65%的80%
然后用20术:目标可以隐形等静洗脱100%甲醇
10分钟.最后,equilibrated柱与等静
65%的甲醇缓冲液为10分钟.
流量是1毫升/分钟.dansylamines的eluted
通过监测紫外吸光度检测在340海里.
结果
27个样本进行抛光啤酒流行
采用高效液相色谱法测定方法.8生物胺:monoamines
他PHM,给出TRP(,)、二胺、钟、计算机辅助设计(CAD),(把
摘要多胺(SPD,以及polyamine-derived次要的
代谢物(PRP,PIR)进行了啤酒
样品.梯度洗脱甲醇在铵
醋酸缓冲区允许洗脱所有的胺在更少
超过30分钟.在测试范围的生物胺浓度
从0.2到10.0毫克/升)的相关系数
峰面积与浓度之间的标准
三聚氰胺是高于0.99每标准
曲线.平均回收率的铵
104.1% 81.6%,以最低的价值为spermine和
为spermidine最高.量化的极限
从建立了新叶asranged毫克/升),为spermidine遗传毫克/升)for2-phenylethylamine 3倍的检测限和介于新叶毫克/升),为spermidine遗传毫克/升)
2-phenylethylamine.